Coding

Part:BBa_K3015013

Designed by: Valentina Stuchlik   Group: iGEM19_BOKU-Vienna   (2019-10-18)


GFP mut3

GFPmut3 is a optimized variant of the GFP from Aequorea victoria that with the following substitutions:
S65G
S72A
Those substitutions will improved folding and excitation at 488nm

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 25
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None